Journal: Bioactive Materials
Article Title: Pretreated exosomes by electrical stimulation accelerate bone regeneration
doi: 10.1016/j.bioactmat.2025.04.019
Figure Lengend Snippet: Proliferation, osteogenic induction and mineralization of ROBs and RBMSCs induced by different types of Elec-exo. A) Schematic representation of the treatment of ROBs and RBMSCs with Norm-exo and Elec-exo (By Figdraw). B). C) Cell proliferation detected by CCK-8 assay, n = 5. D). E) Expression levels of genes associated with osteogenesis. (Bmp2, Col1A1, and Opn) in ROBs and RBMSCs detected by RT-qPCR on day 2, n = 3. F) ALP staining of ROBs (scale bar represents 100 μm) G) Corresponding quantitative analysis of integrated density values, n = 4. H) ARS staining after co-incubation for 21 days (scale bar represents 100 μm) I) Analysis of integrated density values, n = 4. Results are shown as mean ± SD. ns: non-significant differences, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
Article Snippet: After 21 days of treatment, ROBs were stained for osteogenesis using the Osteogenesis Assay Kit (Beyotime, China).
Techniques: CCK-8 Assay, Expressing, Quantitative RT-PCR, Staining, Incubation